Ruben Stepanyan was born in Baku. In 1931, he graduated from the Yerevan State Conservatory in the violin class of D. Lekger, and later in the conducting class of Konstantin Saradzhev in 1936. From 1938 to 1965, Ruben served as a conductor at the Yerevan Opera and Ballet Theatre. During his tenure, he conducted the premieres of various ballets and directed the first production of Yuri Gevorkyan's opera The Bonfire (Kharkuyk) in 1959. Stepanyan was also deeply involved in music education, a career he began in 1929. From 1965 to 1966, Ruben taught at the Gnessin State Musical Pedagogical Institute in Moscow. In 1961, he was honored with the title of Honored Art Worker of Armenia. Ruben Stepanyan passed away in Moscow.